TOV-21G 人卵巢癌細胞

更新時間：2016-08-01 點擊次數：3358

Designations:

TOV-21G

Depositors:

University of Montreal

Biosafety Level:

Shipped:

frozen

Medium & Serum:

See Propagation

Growth Properties:

adherent

Organism:

Homo sapiens deposited as human

Morphology:

epithelial

Source:

Organ: ovary
Tumor Stage: grade 3, stage III
Disease: primary malignant adenocarcinoma; clear cell carcinoma

Cellular Products:

keratin

Permits/Forms:

In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications:

Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24.

Tumorigenic:

Yes

Oncogene:

p53 + (wild type)

DNA Profile (STR):

Amelogenin: X
CSF1PO: 13,15
D13S317: 11,12
D16S539: 10,12
D5S818: 12,13
D7S820: 12
THO1: 7,9.3
TPOX: 8,11
vWA: 17

Cytogenetic Analysis:

47, XX, +10 [49408 ]

Age:

62 years

Gender:

female

Comments:

This cell line was initiated in October of 1991 from a patient of French-Canadian descent with no family history of ovarian cancer.
Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24.

Propagation:

ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C

Subculturing:

Protocol:

Remove and discard culture medium.
Briefly rinse the cell layer with 0.05% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 3 to 4 days

Preservation:

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase

Related Products:

recommended serum:ATCC 30-2020

References:

42090: Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998
49408: Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

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A2780	人卵巢癌細胞	OVAC	卵巢癌細胞
Anglne	人卵巢癌細胞系	NIH:OVCAR-3	人卵巢癌細胞
Caov－3	人乳突狀卵巢腺癌細胞	PA-1	人卵巢畸胎瘤細胞
CHO	中國倉鼠卵巢細胞	Pr-HNV8	菜青蟲卵巢細胞
CHO/dhFr-	倉鼠卵巢細胞,二氫葉酸還原酶缺陷	RP70-a-CHO-0.8MTX	中國倉鼠卵巢細胞
CHO-K1	中國倉鼠卵巢細胞	SF9	晚秋粒蟲卵巢細胞
CHO-pt	轉血小板基因倉鼠卵巢細胞	SKOV3	人卵巢癌細胞
CHO-S	中國倉鼠卵巢懸浮細胞	SKOV-3/DDP	卵巢癌順鉑耐藥株
CoC1	人卵巢癌細胞系	SW1910	人卵巢癌細胞
CoC2	卵巢癌	SW626	人卵巢腺癌細胞
CTLA4 Ig-24	中國倉鼠卵巢細胞	TOV-21G	人卵巢癌
ES-2	人卵巢透明細胞癌	ZA1	轉S9基因倉鼠卵巢細胞
HO-8910	人卵巢癌細胞	ZA6	轉S9基因倉鼠卵巢細胞
HO-8910PM	人高轉移卵巢癌細胞	Cho-K1	倉鼠卵巢細胞亞株
HOC1	卵巢癌細胞	SK-OV-3	人卵巢癌細胞
Lec1	倉鼠卵巢細胞	Sf9	昆蟲卵巢細胞
CHO-K1	倉鼠卵巢細胞亞株	ES-2	人卵巢透明細胞癌
CHO/dhFr-	倉鼠卵巢細胞,二氫葉酸還原酶缺陷	SK-OV-3	人卵巢癌細胞TOV-21G 人卵巢癌細胞TOV-21G 人卵巢