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      當前位置:首頁 >產品中心>細胞庫>人腫瘤細胞、癌細胞>HTB-55Calu-3 人類肺腺癌細胞(胸水)

      Calu-3 人類肺腺癌細胞(胸水)

      簡要描述:Calu-3 人類肺腺癌細胞(胸水)
      上海復祥生物提供各類細胞,代理美國ATCC品牌。

      • 產品型號:HTB-55
      • 廠商性質:生產廠家
      • 更新時間:2025-11-12
      • 訪  問  量:3110

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      Calu-3 人類肺腺癌細胞(胸水)

      ATCC® Number:HTB-55™    

      Designations:Calu-3

      Depositors:J Fogh

      Biosafety Level:1

      Shipped:frozen

      Medium & Serum:See Propagation

      Growth Properties:adherent

      Organism:Homo sapiens (human)

      Morphology:epithelial


      Source:Organ: lung
      Disease: adenocarcinoma
      Derived from metastatic site: pleural effusion


      Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
      Calu-3 人類肺腺癌細胞(胸水)

      Restrictions:The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.

      Applications:transfection host (Roche FuGENE® Transfection Reagents)

      Tumorigenic:Yes

      Antigen Expression:Blood Type A; Rh+

      DNA Profile (STR):Amelogenin: X

      CSF1PO: 11,12

      D13S317: 12

      D16S539: 12,14

      D5S818: 11

      D7S820: 10,11

      THO1: 6,9.3

      TPOX: 8

      vWA: 16,17



      Cytogenetic Analysis:The stemline chromosome number is hypotriploid with the 2S component occurring at 1.4%. Approximay 20 markers were common to most S metaphases, of which i (1p), t(12;?) and t(18;?) were generally paired; t(6t?) had an HSR segment of q arm, and M7 had two secondary constrictions. Normal chromosomes 1, 13, 15 and 17 were absent, and the X was disomic. No Y chromosome was detected in the QM stained preparations.

      Isoenzymes:AK-1, 1

      ES-D, 1

      G6PD, B

      GLO-I, 1-2

      Me-2, 1

      PGM1, 1

      PGM3, 1-2



      Age:25 years

      Gender:male

      Ethnicity:Caucasian

      Comments:The patient had received prior therapy with cytoxan, bleomycin and adriamycin.

      Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
      Atmosphere: air, 95%; carbon dioxide (CO2), 5%
      Temperature: 37.0°C


      Subculturing:Protocol:
      1. Remove and discard culture medium.

      2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

      3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

        Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

      4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

      5. Add appropriate aliquots of the cell suspension to new culture vessels.

      6. Incubate cultures at 37°C.

      Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
      Medium Renewal: 2 to 3 times per week




      MLE-12 小鼠肺上皮細胞

      saos-2 人成骨肉瘤細胞

      MA104 非洲綠猴胚胎腎上皮細胞

      ball-1 細胞

      nci-h226 人肺鱗癌細胞

      MCA38 小鼠結腸癌細胞

      LTPA 小鼠胰腺癌細胞

      NCI-H23 人非小細胞肺癌細胞

      293.00     人胚腎細胞

      293FT    人胚腎細胞-用于慢病毒包裝

      293TN    人胚腎細胞--用于慢病毒包裝

      293AV    人胚腎細胞-用于腺病毒包裝

      293T    人胚腎細胞

      208F    大鼠成纖維細胞

      786-O    人腎透明細胞腺癌細胞

      A172    人腦膠質母細胞瘤細胞

      A549    人肺癌細胞 細胞系購買

      AGS    人胃癌細胞

      BEL7402 人肝癌細胞系

      AZ-521    人胃癌細胞

      B16F0    小鼠黑色素瘤細胞

      Bel-7402    人肝癌細胞

      Bel-7404    人肝癌細胞

      BGC-823    人胃癌細胞

      C6    大鼠腦膠質瘤細胞

      SW480    人結腸腺癌細胞

      CaCo-2    人結腸癌細胞

      Cho-K1    倉鼠卵巢細胞亞株

      COS-7    非洲綠猴SV40轉化的腎細胞

      GES-1    人胃上皮細胞

      HCT-8    人回盲腸癌細胞

      HeLa

      Hepa1-6    小鼠肝癌細胞

      HepG2    人肝癌細胞

      hFOB1.19    人永生化成骨細胞

      HGC-27    人胃癌細胞

      Hs 746T    人胃癌細胞

      HT-29    人結腸癌細胞

      KB    人口腔表皮樣癌細胞

      LoVo    人結腸癌細胞

      LLC-PK1    豬腎近曲小管上皮細胞

      MCF-10A    人正常乳腺上皮細胞

      MCF-7    人乳腺癌細胞

      MDA-MB-231    人乳腺癌細胞

      MGC80-3    人胃癌細胞

      MKN-45    人胃癌細胞

      NCI-H460    人大細胞肺癌細胞

      NIH-3T3    小鼠成纖維細胞

      NUGC-3    人胃癌細胞

      Raw264-7    小鼠單核細胞白血病細胞

      SGC-7901    人胃癌細胞

      SMMC-7721    人肝癌細胞

      SNU-638    人胃癌細胞

      SNU-739    人肝癌細胞

      Tca8113    人舌鱗癌細胞    細胞系購買

      U251    人腦膠質瘤細胞

      U-373MG    人腦神經膠質瘤細胞

      U-87 MG    人腦星形膠質母細胞瘤

      BT-474    人乳腺導管癌細胞

      PANC-1    人胰腺癌細胞

      HEK293    人胚腎細胞

      Hep3B    人肝癌細胞

      HL-7702    人正常肝細胞

      Raw-Blue    小鼠單核細胞白血病細胞

      WEHI-231    小鼠B淋巴細胞

      OPM-2    人骨髓瘤細胞

      NCI-H929    人骨髓瘤細胞

      U266    人骨髓瘤細胞

      EJ    人膀胱癌細胞

      HOC    大鼠肝卵圓細胞

      RWPE-1    人正常前列腺上皮細胞

      CAL 27    人舌鱗癌細胞

      Calu-3    人肺腺癌細胞

      Eca-109    人食管癌細胞

      DLD-1    人結直腸腺癌上皮細胞

      5637.00     人膀胱癌細胞

      SK-OV-3    人卵巢癌細胞

      GBC-SD    人膽囊癌細胞

      BEL7402 人肝癌細胞系

      SW620    人結腸癌細胞

      MRC-5    人胚肺成纖維細胞     細胞系購買

      PC9    人肺癌細胞

      PC14    人肺癌細胞

      BxPC    人原位胰腺腺癌細胞

      PK(15)    豬腎上皮細胞

      MDCK    狗腎上皮細胞

      3D4/21    豬肺泡巨噬細胞

      MHCC-97L    人肝癌細胞(低轉移)

      MHCC-97H    人肝癌細胞系(高轉移)

      MiaCaPa-2    人胰腺癌細胞

      HN13    口腔鱗狀細胞癌細胞

      Bend3    小鼠腦微血管內皮細胞

      QBC939    人膽管癌細胞

      Ishikawa    人子宮內膜癌細胞

      A498    人腎癌細胞

      143B

      WEHI-231    小鼠B淋巴細胞

      H322    人非小細胞肺癌細胞

      Panc O2

      S8    BEL7402 人肝癌細胞系

      R5    細胞系購買

      Pig MSC    豬骨髓間質干細胞

      HCAEC    人冠狀動脈內皮細胞

      marc-145    猴胚胎腎上皮細胞

      3D4/21    豬肺泡巨噬細胞

      PK(15)    豬腎上皮細胞

      MADB-106    大鼠乳腺癌細胞

      Sf9    昆蟲卵巢細胞

      DADY

      HUVEC[HUV-EC-C]    人臍靜脈內皮細胞

      PC14    人肺癌細胞

      PC9    人肺癌細胞

      Eca-109    人食管癌細胞

      CAL 27    人舌鱗癌細胞

      HOC    肝卵圓細胞

      EJ    人膀胱癌細胞

      U266    人骨髓瘤細胞

      293-mTLR5    人胚腎細胞

      Raw-Blue    小鼠單核細胞白血病細胞

      HL-7702    人正常肝細胞

      Hep3B    人肝癌細胞

      U-373MG    人腦神經膠質瘤細胞

      Tca-8113    人舌鱗癌細胞

      SNU-739    人肝癌細胞

      SNU-638    人胃癌細胞

      NUGC-3    人胃癌細胞

      NIH-3T3    小鼠成纖維細胞

      MKN-45    人胃癌細胞

      LLC-PK1    豬腎近曲小管上皮細胞

      Hs 746T    人胃癌細胞

      Hepa1-6    小鼠肝癌細胞

      HCT-8    人回盲腸癌細胞

      BEL7402 人肝癌細胞系

      HaCaT    人永生化表皮細胞

      GES-1    人胃上皮細胞

      B16-F0    小鼠黑色素瘤細胞

      AZ-521    人胃癌細胞

      786-O    人腎透明細胞腺癌細胞

      208F    大鼠成纖維細胞

      293TN    人胚腎細胞--用于慢病毒包裝

      293FT    人胚腎細胞-用于慢病毒包裝

      GP-293    人胚腎細胞

      293-L.P.    人胚腎細胞

      HTB-55 Calu-3 人類肺腺癌 (胸水)
















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