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      當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>EA.hy926人臍靜脈細(xì)胞融合細(xì)胞

      人臍靜脈細(xì)胞融合細(xì)胞

      簡要描述:CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;
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      • 產(chǎn)品型號(hào):EA.hy926
      • 廠商性質(zhì):生產(chǎn)廠家
      • 更新時(shí)間:2025-11-12
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      ATCC® Number: CRL-2922™ Price:

      Designations:

      EA.hy926

      Depositors: CS Edgell

      Biosafety Level: 1

      Shipped: frozen

      Medium & Serum: See Propagation

      Growth Properties: adherent

      Organism: Homo sapiens (human)

      Morphology: endothelial

      CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞

      Source: Tissue: somatic cell hybrid

      Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


      Restrictions: For-profit institutions must obtain license from University of North Carolina, Chapel Hill.

      Antigen Expression: Factor VIII-related antigen; Homo sapiens, expressed

      DNA Profile (STR): Amelogenin: X

      CSF1PO: 10,11,12

      D13S317: 11

      D16S539: 11,12

      D5S818: 11

      D7S820: 8,9,10

      THO1: 6,8,9.3

      TPOX: 8,9

      vWA: 14,17

      CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞

      Comments: The human umbilical vein cell line, EA.hy926, was established by fusing primary human umbilical vein cells with a thioguanine-resistant clone of A549 by exposure to polyethylene glycol (PEG). Hybrid clones were selected in HAT medium and screened for factor VIII-related antigen. EA.hy926 cells have been maintained for more than 100 population doublings (PDLs). Electron photomicrographs demonstrate cytoplasmic distribution of Weibel-Palade bodies and tissue-specific organelles, characteristics of differentiated endothelial cell functions such as angiogenesis, homeostasis/thrombosis, blood pressure and inflammation. [PubMed: 6407019] [PubMed: 2079463]

      Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10.

      Atmosphere: air, 95; carbon dioxide (CO2), 5

      Temperature: 37.0°C

      Subculturing: Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

      1.Remove and discard culture medium.

      2.Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25 (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

      3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

      CRL-2922 EA.hy926

      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

      4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

      5.Transfer cell suspension to a centrifuge tube and spin at approximay 125 X g for 5 to 10 minutes. Discard supernatant.

      6.Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 X 10(3) to 3 X 10(3) viable cells/sq. cm is recommended.

      7.Incubate cultures at 37C. Subculture when cell concentration reaches between 8 X 10(4) and 1 X 10(5) cells/sq. cm.

      Interval: Twice a week

      Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:10 is recommended

      Medium Renewal: Every 2 to 3 days


      Preservation: Freeze medium: Complete Growth medium, 95; DMSO, 5

      Storage temperature: liquid nitrogen vapor phase

      Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

      recommended serum:ATCC 30-2020

      CRL-2922 EA.hy926

      0.25 (w/v) Trypsin - 0.53 mM EDTA in Hank BSS (w/o Ca++, Mg++):ATCC 30-2101

      phosphate-buffered saline:ATCC 30-2200

      Cell culture tested DMSO:ATCC 4-X

      Erythrosin B vital stain solution:ATCC 30-2404

      Trypan Blue vital stain solution:ATCC 30-2402

      References: 92902: Edgell CJ, et al. Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc. Natl. Acad. Sci. USA 80: 3734-3737, 1983. PubMed: 6407019

      92903: Bauer J, et al. In vitro model of angiogenesis using a human endothelium-derived permanent cell line: contributions of induced gene expression, G-proteins, and integrins. J. Cell. Physiol. 153: 437-449, 1992. PubMed: 1280276

      92904: Edgell CJ, et al. Endothelium specific Weibel-Palade bodies in a continuous human cell line, EA.hy926. In Vitro Cell. Dev. Biol. 26: 1167-1172, 1990. PubMed: 2079463

      CRL-2922 EA.hy926

      92905: Rieber AJ, et al. Extent of differentiated gene expression in the human endothelium-derived EA.hy926 cell line. Thromb. Haemostasis 69: 476-480, 1993. PubMed: 8322270






























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