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      當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>MEG-01人成巨核細(xì)胞白血病細(xì)胞

      人成巨核細(xì)胞白血病細(xì)胞

      簡(jiǎn)要描述:CRL-2021 MEG-01 人成巨核細(xì)胞白血病細(xì)胞,
      原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
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      • 產(chǎn)品型號(hào):MEG-01
      • 廠商性質(zhì):生產(chǎn)廠家
      • 更新時(shí)間:2025-11-12
      • 訪(fǎng)  問(wèn)  量:2545

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      CRL-2021 MEG-01 人成巨核細(xì)胞白血病細(xì)胞

      ATCC® Number: CRL-2021™

      Designations: MEG-01

      Depositors: M Tekeuchi, MJ Fitzgerald

      Biosafety Level: 1

      Shipped: frozen

      Medium & Serum: See Propagation

      Growth Properties: mixed, adherent and suspension

      Organism: Homo sapiens (human)

      Morphology: lymphoblast

      CRL-2021 MEG-01 人成巨核細(xì)胞白血病細(xì)胞

      Source: Disease: chronic myelogenous leukemia (CML)

      Cell Type: megakaryoblast;

      Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


      CRL-2021 MEG-01

      Applications: transfection host (Nucleofection technology from Lonza)

      Antigen Expression: CD41 +; CD61 +; CDw14 +

      DNA Profile (STR): Amelogenin: X,Y

      CSF1PO: 10

      D13S317: 8

      D16S539: 9

      D5S818: 13

      D7S820: 11

      THO1: 7

      TPOX: 8,11

      vWA: 16

      Cytogenetic Analysis: hyperdiploid; modal number = 56 to 58; the Philadelphia chromosome (Ph1) is present

      Age: 55 years

      Gender: male

      Comments: The MEG-01 cell line was derived in 1983 at the Nagoya University School of Medicine, Nagoya, Japan from bone marrow cells taken from a patient in megakaryoblastic crisis of CML.

      The cells are positive for cytoplasmic Factor VIII and surface GPIIb/IIIa, periodic acid - Schiff (PAS) reaction, alpha naphthyl acetate esterase and acid phosphatase.

      They are negative for myeloperoxidase, alpha naphthyl butyrate esterase, naphthol AS-D chloroacetate esterase and alkaline phosphatase.

      The stain positively with monoclonal antibodies BA-1 (anti B cell, granulocyte), HPL-3 (anti gpIIb/IIIa) and 20.3 (anti monocyte, plaet).

      They are negative for other lymphoid and myeloid seris antigens.

      Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

      Temperature: 37.0°C

      Subculturing: Protocol: Subcultures are prepared by scraping the adherent cells into the medium. From the resulting suspension dilute cells to a concentration 1-2 X 10exp5 cells/ml into fresh medium in a new flasks. Keep culture below approximate density of 10exp6 cells/ml.

      Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended

      Medium Renewal: 2 to 3 times per week

      Subcultures are prepared by scraping the adherent cells into the medium, and diluting the resulting suspension into fresh medium in new flasks.

      Doubling Time: 36 to 48 hrs

      Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

      recommended serum:ATCC 30-2020

      References: 23348: Ogura M, et al. Establishment of a novel human megakaryoblastic leukemia cell line, MEG- 01, with positive Philadelphia chromosome. Blood 66: 1384-1392, 1985. PubMed: 2998511

      CRL-2021 MEG-01





















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