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      當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>AN3 CA人子宮內(nèi)膜腺癌細(xì)胞

      人子宮內(nèi)膜腺癌細(xì)胞

      簡要描述:HTB-111 AN3 CA 人子宮內(nèi)膜腺癌細(xì)胞,
      原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
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      • 產(chǎn)品型號:AN3 CA
      • 廠商性質(zhì):生產(chǎn)廠家
      • 更新時(shí)間:2025-11-12
      • 訪  問  量:2550

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      HTB-111 AN3 CA 人子宮內(nèi)膜腺癌細(xì)胞

      ATCC® Number: HTB-111™ Price: $399.00

      Designations: AN3 CA

      Depositors: CJ Dawe

      Biosafety Level: 1

      Shipped: frozen

      Medium & Serum: See Propagation

      Growth Proper sapiens (human)

      Morphology: epties: adherent

      Organism: Homoithelial

      HTB-111 AN3 CA 人子宮內(nèi)膜腺癌細(xì)胞

      Source: Organ: uterus

      Tissue: endometrium

      Disease: adenocarcinoma

      Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


      Tumorigenic: Yes

      DNA Profile (STR): Amelogenin: X

      CSF1PO: 12,14,15

      D13S317: 12,14

      D16S539: 10,14

      D5S818: 11,14

      D7S820: 7,10,7.1

      THO1: 10,9.3

      TPOX: 8,10

      vWA: 14,20

      Isoenzymes: AK-1, 1-2

      ES-D, 1

      G6PD, B

      GLO-I, 2

      PGM1, 1

      PGM3, 1-2

      Age: 55 years

      Gender: female

      Ethnicity: Caucasian

      Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

      Atmosphere: air, 95%; carbon dioxide (CO2), 5%

      Temperature: 37.0°C

      Subculturing: Protocol: Volumes used in this protocol are for 75 sq. cm flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

      1.Remove and discard culture medium.

      2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

      3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

      4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

      5.Add appropriate aliquots of the cell suspension to new culture vessels.

      6.Incubate cultures at 37C.

      Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

      Medium Renewal: 2 to 3 times per week

      Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%

      Storage temperature: liquid nitrogen vapor phase

      Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

      recommended serum:ATCC 30-2020

      References: 22517: Dawe CJ, et al. Growth in continuous culture, and in hamsters, of cells from a neoplasma assoicated with Acanthosis nigricans. J. Natl. Cancer Inst. 33: 441-456, 1964. PubMed: 14207855

      22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

      29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105



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