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      當前位置:首頁 >產品中心>細胞庫>小鼠腫瘤細胞、癌細胞>J774A.1 小鼠腹水單核細胞瘤

      J774A.1 小鼠腹水單核細胞瘤

      簡要描述:TIB-67 J774A.1 小鼠腹水單核細胞瘤,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和培養條件

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      TIB-67 J774A.1 小鼠腹水單核細胞瘤,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻i優培養條件

      TIB-67 J774A.1 小鼠腹水單核細胞瘤

      ATCC® Number:TIB-67™  Price:$256.00
      Designations:J774A.1

      Depositors:P Ralph

      Biosafety Level:1

      Shipped:frozen

      Medium & Serum:See Propagation

      Growth Properties:adherent

      Organism:Mus musculus (mouse)

      Morphology:macrophage
      J774A.1 小鼠腹水單核細胞瘤


      Source:Tissue: ascites
      Strain: BALB/cN
      Disease: reticulum cell sarcoma
      Cell Type: monocyte/macrophage macrophage;


      Cellular Products:interleukin 1 beta

      lysozyme [1080]



      Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
      TIB-67

      Isolation:La Jolla California, United States
      Isolation date: 1968


      Applications:Biological response [92560]

      transfection host (Roche FuGENE® Transfection Reagents)



      Receptors:complement (C3), expressed [1135]

      Fc receptor, IgG, high affinity I (Fcgr1), expressed [13710]



      Age:adult

      Gender:female

      Comments:J774A.1 cells are active in antibody dependent phagocytosis [Pubmed: 1101071]. Their growth is inhibited by dextran sulfate, PPD and LPS [Pubmed: 318922]. They synthesize large amounts of lysozyme and exhibits minor cytolysis but predominantly antibody-dependent phagocytosis. Interleukin 1 beta (Il1b) is synthesized continuously by this line.

      Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
      Atmosphere: air, 95%; carbon dioxide (CO2), 5%
      Temperature: 37.0°C


      Subculturing:Protocol: Subcultures are prepared by scraping.

      For a 75 cm2 flask, remove all but 10 ml of the culture medium. (adjust volume accordingly for different culture vessels) Dislodge cells from the flask substrate with a cell scraper, aspirate and dispense into new flasks.


      Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
      Medium Renewal: Replace or add medium two or three times weekly


      Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
      Storage temperature: liquid nitrogen vapor phase


      Doubling Time:17 hours

      Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

      recommended serum:ATCC 30-2020

      purified RNA:ATCC TIB-67R



      References:1080: Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890

      1135: Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

      1136: Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922

      13710: Sears DW, et al. Molecular cloning and expression of the mouse high affinity Fc receptor for IgG1. J. Immunol. 144: 371-378, 1990. PubMed: 2136886

      22827: Ralph P, et al. Reticulum cell sarcoma: an effector cell in antibody-dependent cell- mediated immunity. J. Immunol. 114: 898-905, 1975. PubMed: 1089721

      22896: Ralph P, Nakoinz I. Phagocytosis and cytolysis by a macrophage tumour and its cloned cell line. Nature 257: 393-394, 1975. PubMed: 1101071

      32697: Knowlton KU, et al. A mutation in the puff region of VP2 attenuates the myocarditic phenotype of an infectious cDNA of the woodruff variant of coxsackievirus B3. J. Virol. 70: 7811-7818, 1996. PubMed: 8892902

      32883: Schissel SL, et al. Zn2+-stimulated sphingomyelinase is secreted by many cell types and is a product of the acid sphingomyelinase gene. J. Biol. Chem. 271: 18431-18436, 1996. PubMed: 8702487

      92560: Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.





















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