VERO 非洲綠猴腎細(xì)胞

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VERO C1008 （E6）非洲綠猴腎細(xì)胞

VERO 非洲綠猴腎細(xì)胞的詳細(xì)介紹

ATCC^® Number:

CRL-1586™

Price:

Designations:	VERO C1008 [Vero 76, clone E6, Vero E6]
Depositors:	EM Earley
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Cercopithecus aethiops
Morphology:	epithelial
Source:	Organ: kidney Disease: normal
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Virus Susceptibility:	Junin virus Machupo virus Lassa virus Marburg virus Zaire Ebola virus
Comments:	This is a clone of VERO 76 （ATCC CRL-1587）.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
Subculturing:	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. *Subction Ratio:** A subction ratio of 1:4 is recommended Medium Renewal:* 2 to 3 times per week
Preservation:	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Doubling Time:	22 hours
Related Products:	Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2003 recommended serum:ATCC 30-2020
References:	26095: Earley EM, Johnson KMThe lineage of Vero, Vero 76 and its clone C1008 in the United StatesIn: Earley EM, Johnson KMVero cells: origin, properties and biomedical applicationsTokyoChiba Univ.pp. 26-29, 1988 32579: Schuster FL, Visvesvara GS. Axenic growth and drug sensitivity studies of Balamuthia mandrillaris, an agent of amebic meningoencephalitis in humans and other animals. J. Clin. Microbiol. 34: 385-388, 1996. PubMed: 8789020

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VERO 非洲綠猴腎細(xì)胞

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